The Image Analysis Collaboratory (IAC) at Harvard Medical School started operating in September 2022. We specialize in the quantitative analysis of biomedical images—be that image restoration, object detection, segmentation, co-localization analysis, tracking, classification, or related matters. We apply and develop methods in classical bioimage analysis, deep learning, and spatial statistics—in-house or with the help of our friends and collaborators. We aim to serve as a local/global clearinghouse for bioimage analysis methods, connecting image-data owners with image-analysis practitioners. The core members of the Image Analysis Collaboratory are physicists, mathematicians, computer scientists, engineers, and life scientist with an algorithmic bent: A group of people with diverse backgrounds, united in our desire to extract maximum information from images (in finite time) and interest in teaching best practices.